Beta-2-adaptin Antibody

• Catalog Number: orb1522465
• Category: Antibodies
• Description: Beta-2-adaptin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 575 and 625
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Beta-2-adaptin
• UniProt ID: P63010
• NCBI: NP_001273.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: AP2-BETA; ADTB2; plasma membrane adaptor HA2/AP2 a
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse Beta-2-adaptin by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3, CH27, BW5147.3, CT26, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Beta-2-adaptin antibody (orb1522465) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human Beta-2-adaptin by western blot. Samples: Whole cell lysate (50 µg) from GaMG, HEK293T, U2OS, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Beta-2-adaptin antibody (orb1522465) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human Beta-2-adaptin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Beta-2-adaptin antibody (orb1522465) used for IP at 6 µg per reaction. Beta-2-adaptin was also immunoprecipitated by a previous lot of this antibody (orb1522465) and a second antibody against a different epitope of Beta-2-adaptin.