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Catalog Number | orb1522464 |
---|---|
Category | Antibodies |
Description | Beta-2-adaptin Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 575 and 625 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Beta-2-adaptin |
UniProt ID | P63010 |
NCBI | NP_001273.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | AP2-BETA; ADTB2; plasma membrane adaptor HA2/AP2 a Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of mouse Beta-2-adaptin by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3, CH27, BW5147.3, CT26, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Beta-2-adaptin antibody (orb1522464) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human Beta-2-adaptin by western blot. Samples: Whole cell lysate (50 µg) from GaMG, HEK293T, U2OS, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Beta-2-adaptin antibody (orb1522464) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human Beta-2-adaptin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Beta-2-adaptin antibody (orb1522464) used for IP at 6 µg per reaction. Beta-2-adaptin was also immunoprecipitated by a previous lot of this antibody (orb1522464) and a second antibody against a different epitope of Beta-2-adaptin.
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