beta 1 Adrenergic Receptor antibody

• Catalog Number: orb19192
• Category: Antibodies
• Description: Goat polyclonal antibody to beta 1 Adrenergic Receptor
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: P-ELISA: 1:4000, IHC-P: 4-6μg/ml
• Conjugation: Unconjugated
• MW: 51.2
• Target: ADRB1
• Entrez: 153
• Protein Sequence: ESDEARRCYNDPK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti-ADR B1 antibody, anti-ADRB 1 antibody, anti-A
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 55kDa band observed in Human Heart lysates (calculated MW of 51.2kDa according to NP_000675.1). Recommended concentration: 0.3-1 μg/ml.Experiment Notes: This product has been successfully used in assay standardization (beating frequency) of rat cardiomyocytes (PubMed: Wallkut G. et al., Am Coll Cardiol. 2010 Feb 2;55(5):463-8.; PMID: 20117461). Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb19192 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining.

orb19192 Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.

orb19192 (6µg/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Immunohistochemical staining of Human Heart using beta 1 Adrenergic Receptor antibody.

Immunohistochemical staining of Human Heart using beta 1 Adrenergic Receptor antibody.