Beclin 1 Rabbit Polyclonal Antibody

• Catalog Number: orb704275
• Category: Antibodies
• Description: Beclin 1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human BECN1 (181-280/450aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500, Flow-Cyt=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 60 kDa
• Target: BECN1
• UniProt ID: Q14457
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Beclin-1; ATG6; beclin1; VPS30; Beclin 1 like prot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (orb704275) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (orb704275) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (orb704275) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (orb704275) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Small intestine (Mouse) Lysate at 40 ug, Lane 3: Lung (Mouse) Lysate at 40 ug, Lane 4: Heart (Mouse) Lysate at 40 ug, Lane 5: Cerebrum (Rat) Lysate at 40 ug, Lane 6: Jurkat (Human) Cell Lysate at 30 ug, Lane 7: Raji (Human) Cell Lysate at 30 ug, Lane 8: Hela (Human) Cell Lysate at 30 ug, Lane 9: A431 (Human) Cell Lysate at 30 ug, Lane 10: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-Beclin 1 (orb704275) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55-60 kD, Observed band size: 60 kD.