Beclin 1 Rabbit Polyclonal Antibody

• Catalog Number: orb500808
• Category: Antibodies
• Description: Beclin 1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Porcine
• Reactivity: Human, Mouse, Porcine, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human BECN1 (201-330/450aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 50 kDa
• Target: BECN1
• UniProt ID: Q14457
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Beclin-1; ATG6; beclin1; VPS30; Beclin 1 like prot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Small intestine (Mouse) lysate at 30 ug, Primary: Anti-Beclin-1 (orb500808) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 50kD, Observed band size: 50kD.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (orb500808) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Pig, Primary: Anti-Beclin-1 (orb500808) at 1:500 dilution.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Beclin-1 Polyclonal Antibody, Unconjugated (orb500808) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.