BDNF Antibody

• Catalog Number: orb196246
• Category: Antibodies
• Description: BDNF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Monkey, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: This antibody was raised against E.coli-derived human BDNF recombinant protein (Position: H129-R247). Human BDNF shares 100% amino acid (aa) sequence identity with both mouse and rat BDNF.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat Western blot, 0.1-0.5μg/ml, Mouse, Rat, HumanImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 28123 MW
• UniProt ID: P21237
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Brain-derived neurotrophic factor;BDNF;Bdnf;
• Note: For research use only
• Application notes: WB: The detection limit for BDNF is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U-87 cells using anti-BDNF antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of BDNF using anti-BDNF antibody.Lane 1:Rat Brain Tissue.

IHC analysis of BDNF using anti-BDNF antibody.BDNF was detected in paraffin-embedded section of Rat Brain Tissue.

IHC analysis of BDNF using anti-BDNF antibody.BDNF was detected in paraffin-embedded section of Mouse Brain Tissue.