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Product Overview
Product Name BDNF antibody
Catalog Number orb10181
Species/HostRabbit
ReactivityHuman, Mouse, Rat
Predicted Reactivity Bovine, Canine, Equine, Porcine
ConjugationUnconjugated
Tested applicationsELISA, IF, IHC-P, Flow-Cyt, WB
Immunogen KLH conjugated synthetic peptide derived between 91-170 amino acids of human BDNF
Target BDNF
Alternative Names
Product Properties
Form/Appearance Liquid: 0.01M TBS (pH7.4), 0.5% BSA, 0.015% ProClin™ 300 and 25% glycerol
Concentration - 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Affinity purified by Protein A
MW 13/27 kDa
Uniprot ID P23560 (Human); P21237 (Mouse); P23363 (Rat)
NCBI NP_001137277.11; NM_001143805.11
Entrez 627
Product Description

Rabbit polyclonal antibody to BDNF

Application Notes
Dilution Range WB: 1:500-2000, ELISA: 1:500-1000, IHC-P: 1:400-800, Flow-Cyt: 1, IF: 1:100-500
Validation Images
IHC-P image of mouse skin tissue using BDNF antibody (dilution of primary antibody at 1:100)
IHC-P image of mouse skin tissue using BDNF antibody (dilution of primary antibody at 1:100)
IF image of mouse skin tissue using anti-BDNF (primary antibody at 1:100)
IF image of mouse skin tissue using anti-BDNF (primary antibody at 1:100)
IHC-P staining of mouse skin tissue using BDNF antibody (dilution at 1:100)
IHC-P staining of mouse skin tissue using BDNF antibody (dilution at 1:100)
Immunofluorescence image of mouse skin tissue using BDNF antibody (dilution at 1:100)
Immunofluorescence image of mouse skin tissue using BDNF antibody (dilution at 1:100)
Immunohistochemical staining of mouse skin tissue using BDNF antibody (dilution of primary antibody - 1:100)
Immunohistochemical staining of mouse skin tissue using BDNF antibody (dilution of primary antibody - 1:100)
IHC-P image of pig lung tissue using BDNF antibody (dilution of primary antibody at 1:100)
IHC-P image of pig lung tissue using BDNF antibody (dilution of primary antibody at 1:100)
Immunohistochemical staining of paraffin embedded pig lung tissue using anti-BDNF (primary antibody at 1:100)
Immunohistochemical staining of paraffin embedded pig lung tissue using anti-BDNF (primary antibody at 1:100)
IHC-P staining of pig lung tissue using BDNF antibody (dilution at 1:100)
IHC-P staining of pig lung tissue using BDNF antibody (dilution at 1:100)
Western blot analysis of rat heart (lane 1), lung (lane 2) tissue using BDNF antibody (primary antibody at 1:500)
Western blot analysis of rat heart (lane 1), lung (lane 2) tissue using BDNF antibody (primary antibody at 1:500)
WB analysis of rat heart tissue using BDNF antibody (dilution at 1:500)
WB analysis of rat heart tissue using BDNF antibody (dilution at 1:500)
Western blot analysis of rat brain (lane 1), spinal cord (lane 2) tissue using BDNF antibody (dilution of primary antibody at 1:500)
Western blot analysis of rat brain (lane 1), spinal cord (lane 2) tissue using BDNF antibody (dilution of primary antibody at 1:500)
Immunohistochemical analysis of paraffin-embedded rat brain tissue, using BDNF antibody
Immunohistochemical analysis of paraffin-embedded rat brain tissue, using BDNF antibody
Immunohistochemical analysis of paraffin-embedded rat brain tissue, using BDNF antibody
Immunohistochemical analysis of paraffin-embedded rat brain tissue, using BDNF antibody
Paraffin-embedded rat brain tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). BDNF antibody at 1:200 dilution with overnight incubation at 4 degrees
Paraffin-embedded rat brain tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). BDNF antibody at 1:200 dilution with overnight incubation at 4 degrees
Western blot analysis of rat brain lysate(lane1) and rat heart lysate(Lane2) using BDNF antibody
Western blot analysis of rat brain lysate(lane1) and rat heart lysate(Lane2) using BDNF antibody
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