BCL9L Antibody

• Catalog Number: orb546360
• Category: Antibodies
• Description: BCL9L Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human BCL9L recombinant protein (Position: E397-A666).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 200 kDa
• UniProt ID: Q86UU0
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: B-cell CLL/lymphoma 9-like protein; B-cell lymphom
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-BCL9L antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of BCL9L using anti-BCL9L antibody.Lane 1:human HeLa cell;2:human T-47D cell;3:human PANC-1 cell;4:rat testicular tissue;5:rat liver tissue;6:mouse testicular tissue;7:mouse thymus tissue;8:mouse HEPA1-6 cell.

IF analysis of BCL9L using anti-BCL9L antibody.BCL9L was detected in immunocytochemical section of A431 cell.

IF analysis of BCL9L using anti-BCL9L antibody.BCL9L was detected in immunocytochemical section of A431 cell.

IF analysis of BCL9L using anti-BCL9L antibody.BCL9L was detected in immunocytochemical section of A431 cell.

IHC analysis of BCL9L using anti-BCL9L antibody.BCL9L was detected in paraffin-embedded section of human lung cancer tissues.

IHC analysis of BCL9L using anti-BCL9L antibody.BCL9L was detected in paraffin-embedded section of human Ovarian cancer tissues.

IHC analysis of BCL9L using anti-BCL9L antibody.BCL9L was detected in paraffin-embedded section of rat kidney tissues.

IHC analysis of BCL9L using anti-BCL9L antibody.BCL9L was detected in paraffin-embedded section of human oesophagus squama cancer tissues.

IHC analysis of BCL9L using anti-BCL9L antibody.BCL9L was detected in paraffin-embedded section of human placenta tissues.

IHC analysis of BCL9L using anti-BCL9L antibody.BCL9L was detected in paraffin-embedded section of mouse kidney tissues.