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Catalog Number | orb1672066 |
---|---|
Category | Antibodies |
Description | BCL5 Antibody |
Clonality | Recombinant |
Clone Number | SAIC-23B-22 |
Reactivity | Human |
Isotype | IgG kappa |
Immunogen | Peptide "DGVVEITGK" derived from Heat shock protein beta-1, involved in stress resistance and actin organisation, conjugated to KLH. |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | BCL5 |
UniProt ID | P04792 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | Heat shock protein beta-1, 28 kDa heat shock prote Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence staining of HeLa cells with anti-HSPB1 (SAIC-23B-22). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-23B-22 (orb1672066) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing nucleoli- nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672066- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using the Anti-HSPB1 antibody SAIC-23B-22. Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of SAIC-23B-22 (orb1672066 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Western Blot using anti-HSPB1 antibody SAIC-23B-22. Human heart(A) (0.1 ug/ml) and human placenta(B) (0.3 ug/ml) tissue lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-23B-22 (orb1672066)- before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
FC, ICC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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