BCL6 Antibody

• Catalog Number: orb1252153
• Category: Antibodies
• Description: BCL6 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BCL6/1527
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Amino acids 256-389 were used as the immunogen for the Bcl6 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.125-0.25 ug/mlPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the Bcl6 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: BCL6
• UniProt ID: P41182
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: BCL6 Antibody: BCL5, LAZ3, BCL6A, ZNF51, ZBTB27, B
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.125-0.25 ug/mlPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the Bcl6 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Analysis of HuProt(TM) microarray containing more than 19000 full-length human proteins using Bcl6 antibody (clone BCL6/1527). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE human tonsil tissue with Bcl6 antibody (clone BCL6/1527). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.

IHC testing of FFPE human lymphoma with Bcl6 antibody (clone BCL6/1527). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free Bcl6 Antibody (clone BCL6/1527). Confirmation of Integrity and Purity of the Antibody.