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Catalog Number | orb1728170 |
---|---|
Category | Antibodies |
Description | BCL11B Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human, Rat |
Immunogen | E.coli-derived human BCL11B recombinant protein (Position: Q10-A628). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.25 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg /1x106 cells, Rat Direct ELISA, 0.1-0.5 μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 116 kDa |
UniProt ID | Q9C0K0 |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of C6 cells using anti-BCL11B antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of BCL11B using anti-BCL11B antibody.Lane 1:human MOLT-4 cell.
IF analysis of BCL11B using anti-BCL11B antibody and anti-Tubulin Alpha antibody.BCL11B was detected in immunocytochemical section of U2OS cell.
IHC analysis of BCL11B using anti-BCL11B antibody. BCL11B was detected in a paraffin-embedded section of human bladder urothelial carcinoma with squamous differentiation tissue.
IHC analysis of BCL11B using anti-BCL11B antibody. BCL11B was detected in a paraffin-embedded section of human breast cancer tissue.
IHC analysis of BCL11B using anti-BCL11B antibody. BCL11B was detected in a paraffin-embedded section of human chronic tonsillitis tissue.
IHC analysis of BCL11B using anti-BCL11B antibody. BCL11B was detected in a paraffin-embedded section of human lung adenocarcinoma tissue.
IHC analysis of BCL11B using anti-BCL11B antibody. BCL11B was detected in a paraffin-embedded section of human lymph node metastasis carcinoma tissue.
IHC analysis of BCL11B using anti-BCL11B antibody. BCL11B was detected in a paraffin-embedded section of human placenta tissue.
IHC analysis of BCL11B using anti-BCL11B antibody. BCL11B was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue.
IHC analysis of BCL11B using anti-BCL11B antibody. BCL11B was detected in a paraffin-embedded section of human ovary serous carcinoma tissue.
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