Bcl11b Antibody

• Catalog Number: orb1530279
• Category: Antibodies
• Description: Bcl11b Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 500 and 550
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:5,000 - 1:15,000; IP - 1 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Bcl11b
• UniProt ID: Q9C0K0
• NCBI: NP_612808.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: hRIT1-alpha; ZNF856B; zinc finger protein hRit1 al
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Bcl11b by western blot.Samples: Whole cell lysate from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-Bcl11b antibody orb1530279 at 0.066 µg/ml.

Detection of human Bcl11b by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Bcl11b used at a dilution of 1:1, 000 (1µg/ml).

Detection of human Bcl11b by WB and IP.Samples: Jurkat cells. Antibodies: Affinity purified rabbit anti-Bcl11b antibody used at 0.1 µg/ml (A) and 0.5 µg/ml (B) for WB and at 1 and 10 µg/mg lysate for IP (B).