BCAP31 Antibody

• Catalog Number: orb1239064
• Category: Antibodies
• Description: BCAP31 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-BAP31 antibody (orb1239064) was raised against a peptide corresponding to 16 amino acids near the center of human BAP31. The immunogen is located within amino acids 130 - 180 of BAP31.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-2 μg/mL; IHC: 10 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 28 or 34kDObserved: 28 kD
• Target: BCAP31
• UniProt ID: P51572
• NCBI: NP_005736
• Storage: BAP31 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: BAP31 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: BAP31 Antibody: CDM, DDCH, BAP31, 6C6-AG, DXS1357E
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Rat Lung Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: BAP31, orb1239064 (A: 0.5 µg/mL, B: 1 µg/mL and C: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: BAP31, orb1239064 (0.5 µg/mL), BAP31, (2 µg/mL), and beta-actin orb1240312 (1.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human, Mouse (NIH/3T3) and Rat (YB2/0) Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: BAP31, orb1239064 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of BAP31 in Rat Lung Tissue. Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-BAP31 antibody (orb1239064) at 10 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.