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Catalog Number | orb152189 |
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Category | Antibodies |
Description | Rabbit polyclonal to Bassoon (PerCP). Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntington, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to co-localize with GABA (A) and glutamate (GluR1) receptors.. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | NM_003458.3 (AA 786-1041) N-terminal his-tagged fusion protein |
Concentration | 1 mg/ml |
Dilution range | WB (1:1000), IHC (1:400) |
Conjugation | PerCP |
MW | 420kDa |
Target | Bassoon |
Entrez | 8927 |
UniProt ID | Q9UPA5 |
NCBI | NP_003449.2 |
Storage | Conjugated antibodies should be stored according to the product label |
Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
Alternative names | BSN antibody, ZNF231 antibody, Neuronal double zin Read more... |
Note | For research use only |
Application notes | 1 µg/ml of SPC-198 was sufficient for detection of Bassoon in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry analysis using Rabbit Anti-Bassoon Polyclonal Antibody. Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.
Western blot analysis of Mouse, Rat brain cell lysates showing detection of Bassoon protein using Rabbit Anti-Bassoon Polyclonal Antibody. Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody at 1:1000.
ELISA, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
PerCP |
ELISA, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
PerCP |
IF | |
Human, Rat | |
Mouse | |
Rabbit | |
Polyclonal | |
PerCP/Cy5.5 |
IF | |
Human, Rat | |
Mouse | |
Rabbit | |
Polyclonal | |
PerCP |