Bassoon Antibody

SKU: orb67562

Description

Rabbit Anti-Human Bassoon Polyclonal

Research Area

Disease Biomarkers, Neuroscience

Images & Validation

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Item 1 of 2

Tested Applications	IHC, WB
Dilution range	WB (1:1000), IHC-P (1:400)
Reactivity	Human, Mouse, Rat
Application Notes	1 µg/ml of SPC-198 was sufficient for detection of Bassoon in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	NM_003458.3 (AA 786-1041) N-terminal his-tagged fusion protein
Target	Bassoon
Molecular Weight	420kDa
Purification	Protein A Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Bassoon, BSN, ZNF231, Zinc finger protein 231, Neuronal double zinc finger protein, KIAA0434

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Immunohistochemistry analysis using Rabbit Anti-Bassoon Polyclonal Antibody. Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.

Western blot analysis of Mouse, Rat brain cell lysates showing detection of Bassoon protein using Rabbit Anti-Bassoon Polyclonal Antibody. Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

Bassoon

UniProt

Q9UPA5

NCBI Gene ID

8927

RefSeq (Protein):NP_003449.2

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_003449.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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