Bassoon Antibody (APC)

SKU: orb152184

Description

Rabbit polyclonal to Bassoon (APC). Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntington, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to co-localize with GABA (A) and glutamate (GluR1) receptors..

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB (1:1000), IHC (1:400)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SPC-198 was sufficient for detection of Bassoon in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: NM_003458.3 (AA 786-1041) N-terminal his-tagged fusion protein
• Target: Bassoon
• Molecular Weight: 420kDa
• Purification: Protein A Purified
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Bassoon, BSN, ZNF231, Zinc finger protein 231, Neuronal double zinc finger protein, KIAA0434

Immunohistochemistry analysis using Rabbit Anti-Bassoon Polyclonal Antibody. Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.

Western blot analysis of Mouse, Rat brain cell lysates showing detection of Bassoon protein using Rabbit Anti-Bassoon Polyclonal Antibody. Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

Bassoon

UniProt

Q9UPA5

NCBI Gene ID

8927

RefSeq (Protein):NP_003449.2