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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human BAFF, His Tag at 5 ug/mL (100 uL/well) can bind Human BAFFR, Fc Tag with a linear range of 2-31 ng/mL. |
| Target | BAFFR |
| Tag | Fc Tag |
| MW | 33.2 kDa |
| Purity | >95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Ser 7 - Ala 71 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 33.6 kDa after removal of the signal peptide.The apparent molecular mass of BAFF-R-mFc is approximately 35-55 kDa due to glycosylation.
Mammalian
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Immobilized Human BAFF, His Tag at 5 ug/ml (100 uL/well) can bind Human BAFFR, Fc Tag with a linear range of 2-31 ng/mL.
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Protocol Information
BAFFR Recombinant Protein (orb1227796)
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