BAFF Antibody [Buffy-1]

• Catalog Number: orb1273963
• Category: Antibodies
• Description: BAFF Antibody [Buffy-1]
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: Buffy-1
• Tested applications: FC, ICC, WB
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: BAFF (monoclonal Buffy-1) antibody was raised against recombinant human soluble BAFF (aa. 83-285).
• Concentration: batch dependent
• Dilution range: Clusterin antibody can be used for detection of human clusterin by immunocytochemistry, immunohistochemistry (frozen sections), immunoprecipitation, and western blot (1:2,000). Method: : Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2,000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• UniProt ID: Q9Y275
• Storage: BAFF antibody can be stored at 4°C, stable for one year.
• Buffer/Preservatives: PBS containing 0.02% sodium azide
• Note: For research use only
• Application notes: Clusterin antibody can be used for detection of human clusterin by immunocytochemistry, immunohistochemistry (frozen sections), immunoprecipitation, and western blot (1:2,000). Method: : Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2,000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.
• Expiration Date: 12 months from date of receipt.

The immunogen is recombinant human soluble BAFF, amino acids 83-285. Anti-BAFF (MAb Buffy-1) recognizes the stalk region present in the membrane-bound form of hBAFF. Schematic structure of human soluble BAFF (rec.) (aa 83-285) as seen in this image.