BAFF Antibody [Buffy-1] (FITC)

• Catalog Number: orb1273962
• Category: Antibodies
• Description: BAFF Antibody [Buffy-1] (FITC)
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: Buffy-1
• Tested applications: FC
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: BAFF (monoclonal Buffy-1) antibody was raised against recombinant human soluble BAFF (aa. 83-285).
• Concentration: batch dependent
• Dilution range: BAFF antibody detects endogenous protein by Flow Cytometry. Method: 293T cells (5 x 105) expressing surface BAFF vector or Mock transfected, were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 4 μg/mL of Buffy-1 antibody. After washing in FACS buffer, FITC-conjugated antibody to rat IgG was added and cells were analyzed by flow cytometry.
• Form/Appearance: Liquid
• Conjugation: FITC
• UniProt ID: Q9Y275
• Storage: BAFF antibody can be stored at 4°C, stable for one year.
• Buffer/Preservatives: PBS containing 0.02% sodium azide
• Note: For research use only
• Application notes: BAFF antibody detects endogenous protein by Flow Cytometry. Method: 293T cells (5 x 105) expressing surface BAFF vector or Mock transfected, were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 4 μg/mL of Buffy-1 antibody. After washing in FACS buffer, FITC-conjugated antibody to rat IgG was added and cells were analyzed by flow cytometry.
• Expiration Date: 12 months from date of receipt.

The immunogen is recombinant human soluble BAFF, amino acids 83-285. Anti-BAFF (MAb Buffy-1) recognizes the stalk region present in the membrane-bound form of hBAFF. Schematic structure of human soluble BAFF (rec.) (aa 83-285) as seen in this image.