Bad BH3 Domain Antibody

SKU: orb31287

Description

Rabbit polyclonal antibody to Bad BH3 Domain

Research Area

Cancer Biology, Cell Biology, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB4617
• Immunogen: Synthetic Peptide
• Target: BAD
• Molecular Weight: 18392
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

anti-BAD antibody

Bad BH3 Domain Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of Bad BH3 Domain antibody in mouse bladder tissue lysates (35 ug/lane). Bad (arrow) was detected using the purified Pab.

Quick Database Links

Gene Symbol

BAD

UniProt

Q92934

RefSeq (Protein):NP_004313.1, NP_116784.1