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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. The specific activity is >3. 5 pmoles/min/μg. |
| Target | BACE1 |
| Tag | His Tag |
| MW | 49.2 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Thr 22 - Thr 457 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−BACE1, ASP2, BACE, FLJ90568, HSPC104, KIAA1149, Memapsin-2
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The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 49.3 kDa after removal of the signal peptide. The apparent molecular mass of BACE1-His is approximately 55-100 kDa due to glycosylation.
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Human BACE-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_036236.1 |
|---|
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
BACE1 Recombinant Protein (orb1228176)
- 0.0
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