B7H4 Rabbit Polyclonal Antibody

SKU: orb10163

Description

B7H4 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human B7H4 (50-100/282aa)
• Target: VTCN1
• Molecular Weight: 28 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

B7-H4; B7h4; B7S1; B7x; BC032925; Immune costimulatory protein B7H4; MGC41287; PRO1291; T cell costimulatory molecule B7x; V set domain-containing T cell activation inhibitor 1; VCTN1; VTCN1_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Human lung cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (B7H4) Polyclonal Antibody, Unconjugated (orb10163) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Sample: Lane 1: Mouse Placenta tissue lysates, Lane 2: Mouse Uterus tissue lysates, Lane 3: Rat Uterus tissue lysates, Lane 4: Rat Kidney tissue lysates, Lane 5: Human JEG-3 cell lysates, Lane 6: Human MCF-7 cell lysates, Primary: Anti-B7H4 (orb10163) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 28 kD, Observed band size: 55-65 kD.

Quick Database Links

Gene Symbol

VTCN1

UniProt

Q7Z7D3