You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1523124 |
---|---|
Category | Antibodies |
Description | B7-H3/CD276 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP |
Reactivity | Human |
Immunogen | Between 484 and 534 |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. Use rabbit anti-B7-H3/CD276 antibody A304-367A.; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | B7-H3/CD276 |
UniProt ID | Q5ZPR3 |
NCBI | NP_001019907.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | B7RP-2; CD276 antigen; costimulatory molecule; CD Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human B7-H3/CD276 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-B7-H3/CD276 antibody orb1523124 used for IP at 6 µg per reaction.
Detection of human B7-H3/CD276 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-B7-H3/CD276 (Cat. No. orb1523124) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB Counterstain: IHC hematoxylin
Detection of human B7-H3/CD276 by immunocytochemistry. Samples: FFPE sections of MCF-7 cells (endogenous expression) and CD276 transfected Hek293 cells (over-expression). Antibody: Affinity purified rabbit anti-B7-H3/CD276 (Cat. No. orb1523124) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB
Filter by Rating