B7-H3/CD276 Antibody

• Catalog Number: orb1523123
• Category: Antibodies
• Description: B7-H3/CD276 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: Between 484 and 534
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. Use rabbit anti-B7-H3/CD276 antibody A304-367A.; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: B7-H3/CD276
• UniProt ID: Q5ZPR3
• NCBI: NP_001019907.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: B7RP-2; CD276 antigen; costimulatory molecule; CD
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human B7-H3/CD276 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-B7-H3/CD276 antibody orb1523123 used for IP at 6 µg per reaction.

Detection of human B7-H3/CD276 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-B7-H3/CD276 (Cat. No. orb1523123) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB Counterstain: IHC hematoxylin

Detection of human B7-H3/CD276 by immunocytochemistry. Samples: FFPE sections of MCF-7 cells (endogenous expression) and CD276 transfected Hek293 cells (over-expression). Antibody: Affinity purified rabbit anti-B7-H3/CD276 (Cat. No. orb1523123) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB