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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Target | B7-H3 |
| Tag | His Tag |
| MW | 25.4 kDa |
| Purity | >95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Val 29 - Phe 244 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 24.1 KDa. Observed: 33-45 KDa, reducing conditions
1 mg, 500 μg, 50 μg, 10 μgRecombinant Human B7-H3 Protein [orb2992922]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 50.2 KDa. Observed: 58-85 KDa, reducing conditions
1 mg, 500 μg, 50 μg, 10 μgRecombinant Human B7-H3 Protein (Biotin) [orb2992954]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 49.1 kDa. Observed: 70-95 kDa
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 24.3 KDa. Observed: 38 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human B7-H3 Protein [orb2994418]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 47.3 KDa. Observed: 65-90 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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Mouse B7-H3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Protocol Information
B7-H3 Recombinant Protein (orb1227704)
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