B7-H3 (4Ig) / B7-H3b Recombinant Protein

SKU: orb1231258

Description

B7-H3 (4Ig) / B7-H3b Recombinant Protein

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, WB
• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 48.5 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Source: HEK293 cells
• Target: CD276
• Tag: His Tag
• MW: 48.5 kDa
• Purity: >95% as determined by SDS-PAGE.
• Protein Sequence: Gly 27 - Thr 461

Storage & Handling

• Storage: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

4Ig-B7-H3,B7-H3,CD276,PSEC0249,UNQ309/PRO352,B7 homolog 3

Human B7-H3 (4Ig), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Immobilized Human B7-H3 (4Ig), His Tag at 2 ug/ml (100 uL/well) can bind Monoclonal Anti-Human B7-H3/B7-H3 (4Ig) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL.

Immobilized Monoclonal Anti-Human B7-H3/B7-H3 (4Ig) Antibody, Human IgG1 at 2 ug/ml (100 uL/well) can bind Human B7-H3 (4Ig), His Tag with a linear range of 1-10 ng/mL.

Quick Database Links

Gene Symbol

CD276

UniProt

Q5ZPR3

RefSeq (Protein):NP_001019907.1