cpeA Antibody

SKU: orb344584

Description

B-Phycoerythrin antibody

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes:
  Anti-B-Phycoerythrin antibody has been tested by ELISA, SDS-Page, and dot blot and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Phycoerythrin [Porphyridium creuentum]
• Target: cpeA
• Purity: Anti-B-Phycoerythrin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Phycoerythrin [Porphyridium creuentum].
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-B-Phycoerythrin Antibody, rabbit anti B-Phycoerythrin, rabbit anti Phycoerythrin

Biorbyt Rabbit anti B-Phycoerythrin antibody was used to detect B-Phycoerythrin under reducing (R) conditions. Reduced samples of purified Phycoerythrin contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of anti B-Phycoerythrin antibody (ON 4 C) and detection was using Dylight 649 conjugated Donkey anti rabbit (1:10K 1.5 hr RT in orb348637).

Quick Database Links

Gene Symbol

cpeA

UniProt

P11392