Avidin Antibody

• Catalog Number: orb750608
• Category: Antibodies
• Description: Avidin antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Antiserum
• Conjugation: Unconjugated
• Reactivity: Bacteria
• Form/Appearance: Lyophilized
• Concentration: 80 mg/mL
• Buffer/Preservatives: None
• Purity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Avidin [Hen Egg White]. Cross reactivity against Avidin from other tissues and species may occur but have not been specifically determined.
• Immunogen: Avidin (Hen Egg White)
• UniProt ID: P02701
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.
• Antibody Type: Primary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: rabbit Anti-Avidin antibody, anti-Avidin Egg White
• Note: For research use only

Charge selectivity in phagocytic target binding by macrophages. A-B) Diagrammatic representation of experimental setup (A), where polystyrene microspheres were coated with avidin followed by anti-avidin antibodies and biotinylated polymers. B) Zeta potential measurements of avidin beads and those containing biotinylated poly(L)lysine or heparin. Bars represent means ± SEM. C) Normalized binding index of indicated particles by BMDM.

The glycocalyx of phagocytic targets constitutes a mechanical barrier to ligand recognition and engagement by phagocytic receptors. A) Diagrammatic representation of experimental model. B) Avidin beads opsonized with anti-avidin antibodies and containing biotin-PEG of indicated sizes were incubated with BMDM for 15 min. Normalized binding index is from > 5 fields of 5–10 cells, n = 3. Bars represent means ± SEM.