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ATXN10 Rabbit Polyclonal Antibody (BF647)

Catalog Number: orb1604026

DispatchUsually dispatched within 2-3 weeks
$ 450.00
Catalog Numberorb1604026
CategoryAntibodies
DescriptionATXN10 Rabbit Polyclonal Antibody (BF647)
TargetATXN10
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationBF647
ReactivityMouse, Rat
Predicted ReactivityBovine, Canine, Equine, Human, Porcine, Sheep
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human ATXN10/SCA10 (21-120/475aa)
Tested applicationsFC
Dilution rangeFlow-Cyt=2ug/Test
Antibody TypePrimary Antibody
StorageShipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Alternative namesAtaxin 10; Ataxin-10; ATX10_HUMAN; Atxn10; Brain p
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NoteFor research use only
ATXN10 Rabbit Polyclonal Antibody (BF647)

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-ATXN10 antibody (orb1604026), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.