ATR Antibody

SKU: orb345594

Description

ATR antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:15,000 - 1:70,000, WB: 1:1,000 - 1:5,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 301 kDa in size corresponding to ATR by western blotting in the appropriate cell lysate or extract. Lysates from HeLa, U2OS, RAW264.7 or HEK293 cells are suggested for western blotting. ATR is ubiquitously expressed, with highest expression levels in the testes. Note that isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATR protein.
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human ATR protein. A BLAST analysis was used to suggest cross-reactivity with ATR from human, mouse, rat, monkey, dog, fish and Xenopus sources based on a 100% homology with the immunizing sequence. Cross-reactivity with ATR from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.28 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-ATR antibody, Serine/threonine-protein kinase ATR, Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1, ACTR antibody, AIB1 antibody, amplified in breast cancer 1 antibody, CAGH16 antibody, CBP interacting protein antibody, CTG26 antibody, FRP1, FRP-1, FRP 1

Western blot using Biorbyt's Anti-ATR antibody. Lane 1: HeLa cell nuclear extract (p/n orb348671). Lane 2: HeLa cell nuclear extract pre-incubated with the immunizing peptide (50 µg peptide for 1 h at room temperature followed by centrifugation). Goat serum was used at 5% for blocking. Primary antibody: Anti-ATR at 1:1400 dilution. The arrowhead corresponds to ~301kDa ATR when compared to MW markers (Lane M).

Quick Database Links

UniProt

Q13535

RefSeq:157266317