You have no items in your shopping cart.
Cart summary
Item 1 of 7
Item 1 of 7
ATP7B Antibody (C-term)
Catalog Number: orb1930038
| Catalog Number | orb1930038 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB19038 |
| Tested applications | FC, IF, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:2000, WB: 1:2000, WB: 1:2000, IF: 1:10~50, WB: 1:2000, IHC-P: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 157263 Da |
| Target | This ATP7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1361-1391 amino acids from the C-terminal region of human ATP7B. |
| UniProt ID | P35670 |
| NCBI | NP_001230111.1, NP_000044.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Copper-transporting ATPase 2, Copper pump 2, Wilso Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
ATP7B Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of ATP7B Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
ATP7B Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP7B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Anti-ATP7B Antibody (C-term) at 1:2000 dilution + Rat liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 157 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-ATP7B Antibody (C-term) at 1:2000 dilution + Mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 157 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-ATP7B Antibody (C-term) at 1:2000 dilution + Caco2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 157 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: ATP7B Antibody (C-term) at 1:2000 dilution. Lane 1: Caco2 whole cell lysate. Lane 2: Mouse kidney lysate. Lane 3: Rat liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 157 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
