ATP7A Antibody

• Catalog Number: orb1254254
• Category: Antibodies
• Description: ATP7A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ATP7A (NP_000043.4).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 163kDa
• Target: ATP7A
• UniProt ID: Q04656
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: ATP7A, MK, MNK, DSMAX, SMAX3
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of 293T cells, using ATP7A antibody (orb1254254) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat kidney using ATP7A antibody (orb1254254) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver damage using ATP7A antibody (orb1254254) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using ATP7A antibody (orb1254254) at dilution of 1:100 (40x lens).