ATP5H Antibody

• Catalog Number: orb251511
• Category: Antibodies
• Description: ATP5H Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human ATP5H recombinant protein (Position: A2-L161). Human ATP5H shares 81% and 78% amino acid (aa) sequence identity with mouse and rat ATP5H, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section),2-5μg/ml, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 18491 MW
• UniProt ID: O75947
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: ATP synthase subunit d, mitochondrial;ATPase subun
• Note: For research use only
• Application notes: WB: The detection limit for ATP5H is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of ATP5H using anti-ATP5H antibody.Lane 1:human HeLa cell; 2:human HepG2 cell; 3:human U-87MG cell; 4:human A549 cell; 5:monkey COS-7 cell; 6:human SW620 cell; 7:human Jurkat cell; 8:human 293T cell.

WB analysis of ATP5H using anti-ATP5H antibody.Lane 1:rat brain tissue; 2:rat liver tissue; 3:rat heart tissue; 4:rat PC-12 cell; 5:mouse brain tissue; 6:mouse liver tissue; 7:mouse heart tissue; 8:mouse ANA-1 cell.

IF analysis of ATP5H using anti-ATP5H antibody. ATP5H was detected in an immunocytochemical section of T-47D cells.

IHC analysis of ATP5H using anti-ATP5H antibody. ATP5H was detected in a paraffin-embedded section of human rectal cancer tissue.