ATP5H Antibody

• Catalog Number: orb1270928
• Category: Antibodies
• Description: ATP5H Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This ATP5H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the Central region of human ATP5H.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 18 kDa
• Target: ATP5H
• UniProt ID: O75947
• NCBI: O75947
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: ATP synthase subunit d, mitochondrial, ATPase subu
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

ATP5H antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in HepG2, NCI-H460, MDA-MB453 cell line lysates (35 ug/lane).