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Catalog Number | orb340969 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to ATP5B |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | Recombinant full length protein of human ATP5B |
Dilution range | WB: 1:500-1:2000, IHC-P: 1:50-1:200 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | ATP5B |
Entrez | 506, 11947, 171374 |
UniProt ID | P06576, P56480, P10719 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | ATPMB; ATPSB; ATP synthase subunit beta mitochondr Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of ATP5B expression in SW620 (A), HepG2 (B), mouse kidney (C), mouse brain (D) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 52 kD)
Immunofluorescent analysis of ATP5B staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
FC, IF, IHC-P, WB | |
Bovine, Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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