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Catalog Number | orb1248204 |
---|---|
Category | Antibodies |
Description | ATP5B Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Bovine, Canine |
Reactivity | Human, Mouse, Porcine, Rat |
Immunogen | The immunogen for this antibody is: C-EPIDERGPIKTKQ |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 55kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 56.6kDa according to NP_001677.2). Recommended concentration: 0.3-1ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Approx 55kDa |
Target | ATP5B |
UniProt ID | P06576 |
NCBI | NP_001677.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | ATP5B, ATP synthase, H+ transporting, mitochondria Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 55kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 56.6kDa according to NP_001677.2). Recommended concentration: 0.3-1ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1248204 (0.3 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1248204 (0.3 ug/ml) staining of Pig Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
FC, IF, IHC-P, WB | |
Bovine, Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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