ATP5A1 antibody

• Catalog Number: orb178380
• Category: Antibodies
• Description: Goat polyclonal antibody to ATP5A1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:128000, WB: 0.01-0.03 μg/ml
• Conjugation: Unconjugated
• MW: 59.8; 57.5; 54.5
• Target: ATP5A1
• Entrez: 498
• Protein Sequence: RVHGLRNVQAEE
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ATP synthase, H+ transporting, mitochondrial
• Note: For research use only
• Application notes: WB: Approx 55kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 54.5kDa according to NP_001001935.1). Recommended concentration: 0.01-0.03µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Expiration Date: 12 months from date of receipt.

References

Sikorski, Krzysztof et al. A high-throughput pipeline for validation of antibodies Nat Methods, 15, 909-912 (2018)

PubMed: 30377371

Applications

WB

Western blot analysis of taining of staining of Pig Heart lysates using ATP5A1 antibody.

Western blot analysis of staining of staining of Human (A), fetal Mouse (B), adult Mouse (C) and adult Rat (D) Heart lysates using ATP5A1 antibody.