ATP5A1 Antibody

SKU: orb1248228

Description

ATP5A1 Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 54.5kDa according to NP_001001935.1). Recommended concentration: 0.01-0.03µg/mL.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RVHGLRNVQAEE
• Target: ATP5A1
• Molecular Weight: Approx 55kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATP5A1, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle, ATP5A, ATP5AL2, ATPM, MOM2, OMR, ORM, hATP1, ATP synthase alpha chain, mitochondrial, ATP synthase subunit alpha, mitochondrial, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2, ATP sythase (F1-ATPase) alpha subunit, mitochondrial ATP synthetase, oligomycin-resistant

orb1248228 (0.01 ug/ml) staining of Human (A), fetal Mouse (B), adult Mouse (C) and adult Rat (D) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248228 (0.01 ug/ml) staining of Pig Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

ATP5A1

UniProt

P25705

RefSeq (Protein):NP_001244263.1, NP_004037.1, NP_001001935.1