ATM Antibody

SKU: orb345896

Description

ATM (phospho-S1981) antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:2,000
• Reactivity: Human
• Application Notes:
  Affinity purified sheep anti-ATM has been tested by ELISA and western blotting against native form phosphorylated ATM PK.

Key Properties

• Antibody Type: Primary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This antibody was affinity purified from whole sheep serum prepared by repeated immunizations with a synthetic peptide corresponding to a region near serine 1981 of human ATM conjugated to KLH using maleimide.
• Target: ATM
• Purity: This affinity-purified antibody is directed against human ATM and is useful in determining its presence in various assays. This polyclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts. Reactivity is observed against human ATM and cross reactivity with ATM from other mammalian sources has not been tested.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

sheep anti-ATM pS1981 Antibody, AT mutated antibody, AT protein antibody, AT1 antibody, ATA antibody, Ataxia telangiectasia gene mutated in human beings antibody, Ataxia telangiectasia mutated antibody, ATC antibody

Schematic of ATM induction by DNA damage.

Western Blot of Sheep Anti-ATM pS1981 polyclonal antibody. Lane 1: untreated MCF-7 cell lysate (p/n orb348664). Lane 2: Hydrogen Peroxide stimulated MCF-7 Whole Cell Lysate (p/n orb348670). Load: 35 µg per lane. Primary antibody: ATM pS1981 antibody at 1:1000 for 1 h at room temperature. Secondary antibody: IRDye™800 conjugated Donkey anti-Sheep IgG secondary antibody at 1:5000 for 1h at room temperature. Block: 5% BLOTTO (B501) overnight at 4°C. Predicted/Observed size: 370 kDa, ATM (370 kDa) is indicated by an arrow. Other band(s): ATM splice variants and isoforms.

Quick Database Links

Gene Symbol

ATM

UniProt

Q13315

RefSeq (Protein):NP_000042.3