ATM (phospho-S1981) antibody

• Catalog Number: orb345896
• Category: Antibodies
• Description: ATM (phospho-S1981) antibody
• Species/Host: Sheep
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This antibody was affinity purified from whole sheep serum prepared by repeated immunizations with a synthetic peptide corresponding to a region near serine 1981 of human ATM conjugated to KLH using maleimide.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity-purified antibody is directed against human ATM and is useful in determining its presence in various assays. This polyclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts. Reactivity is observed against human ATM and cross reactivity with ATM from other mammalian sources has not been tested.
• Conjugation: Unconjugated
• UniProt ID: Q13315
• NCBI: NP_000042.3
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: sheep anti-ATM pS1981 Antibody, AT mutated antibod
• Note: For research use only
• Application notes: Affinity purified sheep anti-ATM has been tested by ELISA and western blotting against native form phosphorylated ATM PK.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of untreated MCF-7 cell lysate (Lane1), Hydrogen Peroxide stimulated MCF-7 Whole Cell Lysate (Lane2) using ATM (phospho-S1981) antibody