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Catalog Number | orb345896 |
---|---|
Category | Antibodies |
Description | ATM (phospho-S1981) antibody |
Species/Host | Sheep |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | This antibody was affinity purified from whole sheep serum prepared by repeated immunizations with a synthetic peptide corresponding to a region near serine 1981 of human ATM conjugated to KLH using maleimide. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This affinity-purified antibody is directed against human ATM and is useful in determining its presence in various assays. This polyclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts. Reactivity is observed against human ATM and cross reactivity with ATM from other mammalian sources has not been tested. |
Conjugation | Unconjugated |
UniProt ID | Q13315 |
NCBI | NP_000042.3 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | sheep anti-ATM pS1981 Antibody, AT mutated antibod Read more... |
Note | For research use only |
Application notes | Affinity purified sheep anti-ATM has been tested by ELISA and western blotting against native form phosphorylated ATM PK. |
Expiration Date | 12 months from date of receipt. |
Schematic of ATM induction by DNA damage.
Western Blot of Sheep Anti-ATM pS1981 polyclonal antibody. Lane 1: untreated MCF-7 cell lysate (p/n orb348664). Lane 2: Hydrogen Peroxide stimulated MCF-7 Whole Cell Lysate (p/n orb348670). Load: 35 µg per lane. Primary antibody: ATM pS1981 antibody at 1:1000 for 1 h at room temperature. Secondary antibody: IRDye™800 conjugated Donkey anti-Sheep IgG secondary antibody at 1:5000 for 1 h at room temperature. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 370 kDa, ATM (370 kDa) is indicated by an arrow. Other band(s): ATM splice variants and isoforms.
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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