ATG9B Antibody

• Catalog Number: orb384106
• Category: Antibodies
• Description: Rabbit Anti-Human ATG9B Polyclonal
• Target: ATG9B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Purification: Peptide Affinity Purified
• Immunogen: Synthetic peptide from the N-terminal of Human ATG9B (aa. 110-121)
• UniProt ID: Q674R7
• MW: 100 kDa
• Tested applications: ICC, IF, WB
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Application notes: A 1:1000 dilution of SPC-646 was sufficient for detection of ATG9B in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: APG9-like 2 Antibody, APG9L2 Antibody, Nitric oxid
• Note: For research use only
• Entrez: 285973
• NCBI: NP_001303985.1

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG9B Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome Membrane, Multi-Pass Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG9B Antibody (D) Composite.

Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 μg. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.