ATG9B Antibody: Biotin

• Catalog Number: orb384117
• Category: Antibodies
• Description: Rabbit polyclonal antibody against ATG9B conjugated to Biotin.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse
• Immunogen: Synthetic peptide from the N-terminal of Human ATG9B (aa. 110-121)
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Conjugation: Biotin
• MW: 100 kDa
• Target: ATG9B
• Entrez: 285973
• UniProt ID: Q674R7
• NCBI: NP_001303985.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: APG9-like 2 antibody, APG9L2 antibody, Nitric oxid
• Note: For research use only
• Application notes: A 1:1000 dilution of SPC-646 was sufficient for detection of ATG9B in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG9B Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome Membrane, Multi-Pass Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG9B Antibody (D) Composite.

Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 μg. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.