ATG9B Antibody (Biotin)

SKU: orb384117

Description

Rabbit polyclonal antibody against ATG9B conjugated to Biotin.

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse
• Application Notes:
  A 1:1000 dilution of SPC-646 was sufficient for detection of ATG9B in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the N-terminal of Human ATG9B (aa. 110-121)
• Target: ATG9B
• Molecular Weight: 100 kDa
• Purification: Peptide Affinity Purified
• Conjugation: Biotin

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

ATG9B, ATG9B_HUMAN, APG9L2, APG9-like 2, Autophagy-related protein 9B, Protein sONE, NOS3AS, Nitric oxide synthase 3-overlapping antisense gene protein

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG9B Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome Membrane, Multi-Pass Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG9B Antibody (D) Composite.

Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 μg. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.

Quick Database Links

Gene Symbol

ATG9B

UniProt

Q674R7

NCBI Gene ID

285973

RefSeq (Protein):NP_001303985.1