ATG5/APG5L Rabbit Polyclonal Antibody

• Catalog Number: orb1152149
• Category: Antibodies
• Description: ATG5/APG5L Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Porcine, Sheep
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ATG5/APG5L (1-100/275aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 32 kDa
• Target: ATG5
• UniProt ID: Q9H1Y0
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: APG 5; APG 5L; APG5; APG5 autophagy 5 like; APG5 l
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ATG5/APG5L) Polyclonal Antibody, Unconjugated (orb1152149) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ATG5/APG5L) Polyclonal Antibody, Unconjugated (orb1152149) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Stomach tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Primary: Anti-ATG5/APG5L (orb1152149) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 32 kDa, Observed band size: 50 kDa.