ATG5 Antibody

SKU: orb1239087

Description

ATG5 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Porcine
• Application Notes:
  ATG5 antibody can be used for the detection of ATG5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: ATG5 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG5.The immunogen is located within the first 50 amino acids of ATG5.
• Target: ATG5
• Purification: ATG5 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: ATG5 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATG5 Antibody: ASP, APG5, APG5L, hAPG5, APG5-LIKE

Western blot analysis of ATG5 in rat spleen tissue lysate with ATG5 antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of ATG5 in human spleen tissue with ATG5 antibody at 2.5 µg/mL.

Immunofluorescence of ATG5 in Human Spleen cells with ATG5 antibody at 20 µg/mL.

Immunofluorescence of ATG5 in Hela cells with ATG5 Antibody at 5 µg/ml.

Quick Database Links

Gene Symbol

ATG5

UniProt

A9UGY9

RefSeq:EAW48415