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ATG4D Antibody, KO Validated
Catalog Number: orb1274280
| Catalog Number | orb1274280 |
|---|---|
| Category | Antibodies |
| Description | ATG4D Antibody, KO Validated |
| Target | ATG4D |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | batch dependent |
| Buffer/Preservatives | Liquid |
| Purification | Affinity purification |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ATG4D (NP_116274.3). |
| UniProt ID | Q86TL0 |
| MW | Observed: 53kDa |
| Tested applications | IF, WB |
| Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Autophagy related 4D cysteine peptidase, APG4D, AP Read more... |
| Note | For research use only |
Western blot analysis of extracts from normal (control) and ATG4D knockout (KO) HeLa cells, using ATG4D antibody (orb1274280) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
