ATG4D Antibody (C-term)

• Catalog Number: orb1933457
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This ATG4D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 441-470 amino acids from the C-terminal region of human ATG4D.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q86TL0
• MW: 52922 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:100, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Antibody Type: Primary Antibody
• Clone Number: RB7567
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cysteine protease ATG4D, 3422-, AUT-like 4 cystein
• Note: For research use only
• NCBI: NP_116274.3, NP_001268433.1

Western blot analysis of APG4D Pab in Y79 cell lysate.

Cos7, HEK293, MEF, and Hela cells, left to right respectively.

Fluorescent image of U251 cells stained with ATG4D (C-term) antibody. U251 cells were treated with Chloroquine (50 μM, 16 h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ATG4D (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG4D immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.