ATG1/ULK1 Rabbit Polyclonal Antibody

SKU: orb5730

Description

ATG1/ULK1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=0.5μg /Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Equine, Gallus, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ATG1/ULK1 (951-1050/1050aa)
• Target: ULK1
• Molecular Weight: 116 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ATG 1; ULK1; ATG1; ATG1A; Serine/threonine protein kinase ULK1; Serine/threonine protein kinase Unc51.1; ULK 1; Unc 51(C. elegans) like kinase 1; UNC 51; Unc 51 like kinase 1; Unc-51-like kinase 1; Unc-51 like kinase 1(C. elegans); UNC51; ULK1_HUMAN; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; hATG1.

Positive control: RSC96, Isotype Control Antibody: Rabbit IgG, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody catalog number: orb5730, dilution: 0.5 µg in 100 µl 1X PBS containing 0.5% BSA.

Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-ATG1/ULK1 (orb5730) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 116 kD, Observed band size: 116 kD.

SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (ATG1/ULK1) polyclonal Antibody, Unconjugated (orb5730) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: mouse pancreas tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-ATG1/ULK1 Polyclonal Antibody, Unconjugated (orb5730) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

ULK1

UniProt

O75385