ATG1/ULK1 Rabbit Polyclonal Antibody

• Catalog Number: orb5730
• Category: Antibodies
• Description: ATG1/ULK1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ATG1/ULK1 (951-1050/1050aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=0.5μg /Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 116 kDa
• Target: ULK1
• UniProt ID: O75385
• RRID: AB_10933012
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ATG 1; ULK1; ATG1; ATG1A; Serine/threonine protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Positive control: RSC96, Isotype Control Antibody: Rabbit IgG, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody catalog number: orb5730, dilution: 0.5 µg in 100 µl 1X PBS containing 0.5% BSA.

Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-ATG1/ULK1 (orb5730) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 116 kD, Observed band size: 116 kD.

SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (ATG1/ULK1) polyclonal Antibody, Unconjugated (orb5730) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: mouse pancreas tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-ATG1/ULK1 Polyclonal Antibody, Unconjugated (orb5730) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.