ATG12 Antibody

SKU: orb153327

Description

Goat polyclonal antibody to ATG12 - autophagosome marker. Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB:1:500-1:2,000, IF:1:50-1:250, IHC-P:1:200-1:1,000, IHC-F:1:200-1:1,000
• Reactivity: Bovine, Canine, Donkey, Equine, Feline, Gallus, Goat, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Primate, Rabbit, Rat, Sheep
• Application Notes:
  The antibody solution should be gently mixed before use.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Antigen: Purified recombinant peptide within residues 65 aa to the N-terminus of human ATG12 produced in E. coli.. Antigen Sequence: AEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGTEEPAGD
• Target: Autophagy related 12
• Purification: Epitope affinity purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 20% glycerol and 0.05% sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

APG12, Apg12 (autophagy, yeast) homolog, APG12L, FBR93, HAPG12, autophagy protein 12, autophagy-related protein 12 antibody.

Western blot analysis of HEK293 cell line lysate using ATG12 antibody.

Confocal immunofluoroscence analysis of Hepa1-6 cells using ATG12 antibody.

Quick Database Links

Gene Symbol

Autophagy related 12