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Catalog Number | orb153327 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to ATG12 - autophagosome marker. Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy. |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Reactivity | Bovine, Canine, Donkey, Equine, Feline, Gallus, Goat, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Primate, Rabbit, Rat, Sheep |
Isotype | IgG |
Immunogen | Purified recombinant peptide within residues 65 aa to the N-terminus of human ATG12 produced in E. coli. |
Concentration | 1 mg/ml |
Dilution range | WB:1:500-1:2,000, IF:1:50-1:250, IHC-P:1:200-1:1,000, IHC-F:1:200-1:1,000 |
Conjugation | Unconjugated |
Target | ATG12 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS, 20% glycerol and 0.05% sodium azide |
Alternative names | APG12, Apg12 (autophagy, yeast) homolog, APG12L, F Read more... |
Note | For research use only |
Application notes | The antibody solution should be gently mixed before use. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of HEK293 cell line lysate using ATG12 antibody.
Confocal immunofluoroscence analysis of Hepa1-6 cells using ATG12 antibody.
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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