ATG12 antibody

• Catalog Number: orb153327
• Category: Antibodies
• Description: Goat polyclonal antibody to ATG12 - autophagosome marker. Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Bovine, Canine, Donkey, Equine, Feline, Gallus, Goat, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Primate, Rabbit, Rat, Sheep
• Isotype: IgG
• Immunogen: Purified recombinant peptide within residues 65 aa to the N-terminus of human ATG12 produced in E. coli.
• Concentration: 3 mg/ml
• Dilution range: WB:1:500-1:2,000, IF:1:50-1:250, IHC-P:1:200-1:1,000, IHC-F:1:200-1:1,000
• Form/Appearance: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
• Conjugation: Unconjugated
• Target: ATG12
• Storage: Store at -20°C for long-term storage. Store at 2-8°C for up to one month.
• Buffer/Preservatives: PBS, 20% glycerol and 0.05% sodium azide
• Alternative names: APG12, Apg12 (autophagy, yeast) homolog, APG12L, F
• Note: For research use only
• Application notes: The antibody solution should be gently mixed before use.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HEK293 cell line lysate using ATG12 antibody.

Confocal immunofluoroscence analysis of Hepa1-6 cells using ATG12 antibody.