Atf4 Rabbit Polyclonal Antibody

SKU: orb576181

Description

Rabbit polyclonal antibody to Atf4

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Rat, Sheep

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse
• Target: Atf4
• Molecular Weight: 39 kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

Atf-, C/AT, CREB, Atf-4, C/ATF, CREB2, CREB-2, TAXREB, TAXREB67

25 ug of the indicated Mouse tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. An isoform containing the peptide sequence is present at ~46 kDa. Protein is modified by phosphorylation.

Sample Tissue: Mouse Brain, Antibody Dilution: 1 ug/ml.

Sample Tissue: Mouse Mouse Spleen, Antibody Dilution: 2 ug/ml.

Mouse organ of Corti labeled with orb576181 (anti-ATF4) in green, rhodamine phalloidin in red and DAPI in blue. Antibody dilution: 1:100.

WB Suggested Anti-Atf4 Antibody Titration: 0.2-1 ug/ml, Positive Control: Mouse Brain.

Quick Database Links

Gene Symbol

Atf4

UniProt

Q5U4B2

RefSeq (Protein):NP_033846