ATDC antibody

• Catalog Number: orb345654
• Category: Antibodies
• Description: ATDC antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-ATDC affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of human ATDC protein around lysine 116.
• Concentration: 0.67 mg/mL
• Dilution range: ELISA: 1:100,000, WB: 1:500 to 3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-ATDC antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with over-expressed, acetylated and non-acetylated (at K116) ATDC protein. A BLAST analysis was used to suggest cross-reactivity with ATDC from human, horse, bovine, chimpanzee and macaque based on a 100% homology with the immunizing sequence. Partial reactivity is expected against rat and mouse ATDC based on 92% homology with the immunizing sequence. Cross-reactivity with ATDC from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q14134
• NCBI: 17402909
• Storage: Store anti-ATDC Antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-ATDC Antibody, Ataxia-telangiectasia g
• Note: For research use only
• Application notes: Anti-ATDC affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity and detection of ATDC should be optimized by the end user. Expect a band approximately ~66 kDa in size corresponding to ATDC by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of dot blot with acetylated and non-acetylated forms of the immunizing peptide using ATDC antibody