ATDC Ac-K116 Antibody

SKU: orb345656

Description

ATDC Ac-K116 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:100,000, WB: 1:500 to 3,000
• Reactivity: Bovine, Equine, Human, Monkey, Primate
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity and detection of ATDC Ac-K116 should be optimized by the end user. Expect a band approximately ~66 kDa in size corresponding to ATDC Ac-K116 by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of human ATDC protein around lysine 116.
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with over-expressed, acetylated lysine ATDC protein. A BLAST analysis was used to suggest cross-reactivity with ATDC from human, horse, cattle, chimpanzee and macaque based on a 100% homology with the immunizing sequence. Partial reactivity is expected against rat and mouse ATDC based on 92% homology with the immunizing sequence. Cross-reactivity with ATDC from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 0.67 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-ATDC Ac-K116 Antibody, rabbit anti-ATDC acetylated Lys116 Antibody, Ataxia-telangiectasia group D associated protein antibody, FLJ36085 antibody, TRIM 29 antibody, Tripartite motif containing protein 29 antibody, TRIM-29

Western blot using Biorbyt's affinity purified anti-ATDC (Ac-K116) antibody shows detection of a 66 kDa band corresponding to over-expressed, acetylated lysine (K116) ATDC (arrowhead) in transfected 293T cells. No staining is noted for cells transfected with empty vector only. No staining is noted for cells transfected with an ATDC K116R mutant (K to R transversion lacks site for acetylation). In each instance, samples were prepared with and without TSA (1.3uM, 6 hr) which inhibits deacetylation.

Quick Database Links

UniProt

Q14134

RefSeq:17402909